Performing loss-of-function screens with CRISPR-generated libraries has many advantages over using RNAi libraries including the ability to create stable and inheritable genome alterations, complete knockout instead of partially inhibition of gene function, and clear-cut results.
Creating stable and inheritable gene knockouts allows analysis of key phenotypes that may take a longer period of time to manifest.
Because generating and validating genome-edited cell lines can take weeks to several months, Edi Gene is committed to being transparent through the entire process and will provide regular progress reports (every 2 -3 weeks) so you may monitor and stay abreast of the process.
High Throughput Genetic Screening: Edi Gene integrates CRISPR/Cas9 genome editing and high throughput loss of function genetic screening to accelerate new drug target discovery and further clarify mechanisms of action.
You may choose from one or more of over 2000 (and rapidly growing! Unlike other ready-made CRISPR-generated knockout cell lines, Edi Gene’s knockout cell lines are widely used cell lines (He La and 293T) that are more likely to be applicable to your studies.
CRISPR Genome Editing Tools: Edi Gene provides you with the flexibility to perform the CRISPR/Cas9 genome editing that suits your applications.
As most of you are aware, the soon to be replaced USP Heavy Metals Limit test has been in existence for over 100 years.
Learn for yourself why “Edi Gene KO Validated” is rapidly becoming the industry gold standard for validating high quality polyclonal and monoclonal antibodies.
Elemental impurities in pharmaceutical products can be introduced from a number of sources, including catalysts, raw materials, production processes, and packaging materials.
The implementation of the new USP chapters Ideal circumstances the sample under investigation is in a liquid form, so it can be diluted in an aqueous or organic solvent or aspirated without any prior sample preparation.
Edi Gene will design efficient sg RNA and donor DNA, determine and utilize the best transfection method for your cell type, screen and perform single-cell clonal selection.
The edited genome will be thoroughly validated via PCR analysis and Sanger sequencing.
However, if the sample is a solid or powdered material, chances are that it will have to be digested either via an open-vessel hot plate dissolution technique using concentrated mineral acids, or with a closed-vessel, microwave digestion procedure.